Journal article
RhopH2 and RhopH3 export enables assembly of the RhopH complex on P. falciparum-infected erythrocyte membranes
M Pasternak, JMJ Verhoef, W Wong, T Triglia, MJ Mlodzianoski, N Geoghegan, C Evelyn, AZ Wardak, K Rogers, AF Cowman
Communications Biology | NATURE PORTFOLIO | Published : 2022
Abstract
RhopH complexes consists of Clag3, RhopH2 and RhopH3 and are essential for growth of Plasmodium falciparum inside infected erythrocytes. Proteins are released from rhoptry organelles during merozoite invasion and trafficked to the surface of infected erythrocytes and enable uptake of nutrients. RhopH3, unlike other RhopH proteins, is required for parasite invasion, suggesting some cellular processes RhopH proteins function as single players rather than a complex. We show the RhopH complex has not formed during merozoite invasion. Clag3 is directly released into the host cell cytoplasm, whilst RhopH2 and RhopH3 are released into the nascent parasitophorous vacuole. Export of RhopH2 and RhopH3..
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Grants
Awarded by State Government of Victoria
Funding Acknowledgements
We thank the Red Cross Blood Service (Melbourne, Australia) for supply of donor blood for our cell culture. We thank David Steer from Monash Biomedical Proteomics Facility for processing and analysing mass spectrometry samples. The lattice light-sheet referenced in this research was used under license from Howard Hughes Medical Institute, Janelia Research Campus. This work was supported by the National Health and Medical Research Council of Australia (Grants 637406, 1010326, 1049811 and 1057960) and a Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. M.P. was supported by an EMBO Long Term Fellowship ALTF 793-2016 and Sir Henry Wellcome Fellowship 206515_Z_17_Z. J.M.J.V. was supported by the NBS travel grant by the Nora Baart Foundation and scholarships from Radboud University and Radboudumc.